Reduning Attenuates LPS-Induced Human Unmilical Vein Endothelial Cells (HUVECs) Apoptosis Through PI3K-AKT Signaling Pathway

• DOI: 10.3389/fphar.2022.921337
• Publication Date: 2022-07-12T07:29:34Z
• Authors: Ziyi Wang; Xuesong Wang; Zhe Guo; Haiyan Liao; Yan Chai; Ziwen Wang; Zhong Wang
• Keywords: KEGG

The molecular mechanism of Reduning (RDN) in the treatment sepsis was analyzed based on network pharmacology. system pharmacology method administered to search active ingredients and targets RDN, identify sepsis-related genes, determine RDN sepsis. Cytoscape used build a "drug component-target" screen key compounds. A protein-protein interaction (PPI) constructed using STRING, core were revealed through topological analysis. 404 shared introduced into DAVID Bioinformatics Resources 6.8 for GO KEGG enrichment analysis predict their possible signaling pathways explore mechanisms. highlighted that they largely related protein phosphorylation, inflammatory reaction, positive regulation mitogen-activated kinase (MAPK) cascade. outlined enriched PI3K-AKT pathway, calcium rhoptry-associated 1 (Rap1) advanced glycation end products receptors (AGE-RAGE) pathway. Molecular biological validation results exposed could significantly improve expression p-AKT p-PI3K, alleviate apoptosis-related proteins level decrease apoptosis rate LPS-induced HUVECs. In conclusion, it illustrated considerably constrain by activating which advocated basis fundamental research clinical application