PM2.5 Synergizes With Pseudomonas aeruginosa to Suppress Alveolar Macrophage Function in Mice Through the mTOR Pathway

• DOI: 10.3389/fphar.2022.924242
• Publication Date: 2022-06-21T05:59:02Z
• Authors: Jianlong Zhang; Chong Liu; Guangrong Zhao; Meng Li; Di Ma; Qingguo Meng; Wenli Tang; Qingrong Huang; Peimin Shi; Youzhi Li; Linlin Jiang; Xin Yu; Hongwei Zhu; Guozhong Chen; Xingxiao Zhang
• Keywords: Alveolar macrophage

High concentrations of PM2.5 in enclosed broiler houses cause respiratory disorders humans and animals. Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen that can induce severe disease animals under stress or with abnormal immune functions. Alveolar macrophages are lung-resident cells play important roles lung host defence balance. In this study, the mechanism by which synergizes P. to damage alveolar macrophage function inflammation was investigated. The results will provide a theoretical basis for improving poultry breeding environment preventing recurrence infection aeruginosa. were stimulated collected house Phagocytosis determined neutral red test. apoptosis rate cytoskeleton changes observed flow cytometry assays laser scanning confocal microscopy. Protein levels related autophagy mTOR pathway detected Western blotting. indicated combination could decrease phagocytosis, inhibit autophagy, increase apoptosis, destroy macrophages. addition, had significantly increased expression pathway-related proteins response synergistic stimulation above confirmed synergized impede caused more system injuries through process closely activation signalling pathway.