Diosgenin normalization of disrupted behavioral and central neurochemical activity after single prolonged stress

• DOI: 10.3389/fphar.2023.1232088
• Publication Date: 2023-08-17T13:31:56Z
• Authors: Hurmat Malik; Muhammad Usman; Mehreen Arif; Zainab Ahmed; Gowhar Ali; Khalid Rauf; Robert D. E. Sewell
• Keywords: Diosgenin; Neurochemical; Corticosterone; Neurochemistry

Introduction: Post-traumatic stress disorder (PTSD) is a chronic mental illness triggered by traumatic experiences such as wars, natural disasters, or catastrophes, and it characterized anxiety, depression cognitive impairment. Diosgenin steroidal sapogenin with known neuroprotective antioxidant properties. This study aimed to assess the pharmacological potential of diosgenin in single prolonged (SPS) model PTSD, plus other behavioral models along any consequent alterations brain neurochemistry male mice. Methodology: SPS was induced restraining animals for 2 h, followed 20 min forced swim, recuperation 15 min, finally, exposure ether induce anesthesia. The SPS-exposed were treated (20, 40, 60 mg/kg) compared positive controls, fluoxetine donepezil, then they observed changes anxiety/depression-like behaviors, After screening, postmortem serotonin, noradrenaline, dopamine, vitamin C, adenosine its metabolites inosine hypoxanthine quantified frontal cortex, hippocampus, striatum high-performance liquid chromatography. Additionally, animal serum screened corticosterone levels. Results: results showed that reversed anxiety- depression-like ameliorated impairment dose-dependent manner. restored monoamine C levels dose-dependently modulated regions. also reinstated otherwise increased Conclusion: findings suggest may be candidate improving symptoms PTSD.