Luteolin inhibits GPVI-mediated platelet activation, oxidative stress, and thrombosis

• DOI: 10.3389/fphar.2023.1255069
• Publication Date: 2023-10-31T11:30:14Z
• Authors: Yujia Ye; Lihong Yang; Min Leng; Qian Wang; Jiankui Wu; Wen Wan; Huawei Wang; Longjun Li; Yunzhu Peng; Shengjie Chai; Zhaohui Meng
• Keywords: GPVI

Introduction: Luteolin inhibits platelet activation and thrombus formation, but the mechanisms are unclear. This study investigated effects of luteolin on GPVI-mediated in vitro explored effect thrombosis, coagulation, production vivo. Methods: Washed human platelets were used for aggregation, membrane protein expression, ATP, Ca2+, LDH release, adhesion/spreading, clot retraction experiments. to detect collagen convulxin-induced reactive oxygen species endogenous antioxidant effects. C57BL/6 male mice ferric chloride-induced mesenteric collagen-epinephrine induced acute pulmonary embolism, tail bleeding, coagulation function, toxicity The interaction between GPVI was analyzed using solid phase binding assay surface plasmon resonance (SPR). Results: inhibited collagen- convulxin-mediated adhesion, release. ROS increased capacity. reduced ITAM MAPK signaling molecules. Molecular docking simulation showed that forms hydrogen bonds with GPVI. Surface bonded integrin αIIbβ3-mediated activation. artery thrombosis adrenergic-induced mice. decreased oxidative stress did not affect hemostasis, or Discussion: may be an effective safe antiplatelet agent target A new mechanism (decreased stress) anti-platelet activity has been identified.