Large chemokine binding spectrum of human and mouse atypical chemokine receptor GPR182 (ACKR5)

• DOI: 10.3389/fphar.2023.1297596
• Publication Date: 2023-11-01T11:17:33Z
• Authors: Remy Bonnavion; Shangmin Liu; Haruya Kawase; Kenneth Anthony R...; Stefan Offermanns
• Keywords: CCL21; CCL17; XCL2; CCL19; CXCL13; CCL25; CCL13; CCL22; CXCL2; CXCL14; CCL7; CCL5; CC chemokine receptors

Atypical chemokine receptors (ACKRs) play pivotal roles in immune regulation by binding chemokines and regulating their spatial distribution without inducing G-protein activation. Recently, GPR182, provisionally named ACKR5, was identified as a novel ACKR expressed microvascular lymphatic endothelial cells, with functions hematopoietic stem cell homeostasis. Here, we comprehensively investigated the profile of human mouse GPR182. Competitive assays using flow cytometry revealed that besides CXCL10, CXCL12 CXCL13, also CXCL11, CXCL14 CCL25, well CCL1, CCL11, CCL19, CCL26, XCL1 CCL22, CCL24, CCL27 CCL28 bind an affinity less than 100 nM to In line observed vitro, elevated serum levels were GPR182-deficient mice, underscoring role GPR182 scavenging. These data show broader repertoire previously reported they will be important for future work exploring physiological pathophysiological which propose renamed atypical receptor 5 (ACKR5).