Role of symbiotic microbiota dysbiosis in the progression of chronic kidney disease accompanied with vascular calcification

• DOI: 10.3389/fphar.2023.1306125
• Publication Date: 2024-01-05T04:18:52Z
• Authors: Mengying Sun; Yilong Fang; Jianhua Zheng; Guojuan Shi; Junli Guo; Xinzhou Zhang; Rui Zhang
• Keywords: Pharmacology; 0301 basic medicine; 03 medical and health sciences; gut microbiota; Acinetobacter; vascular calcification; blood microbiota; Therapeutics. Pharmacology; RM1-950; chronic kidney disease

Background: Chronic kidney disease (CKD) is now globally recognized as a critical public health concern. Vascular calcification (VC) represents significant risk factor for cardiovascular events in individuals with CKD. It the accessible and precise diagnostic biomarkers monitoring progression of CKD concurrent VC are urgently needed. Methods: The adenine diet-induced rat model was utilized to investigate chronic injury, thoracic aorta, dysregulation biochemical indices. Enzyme-linked immune sandwich assays were employed analyze changes calcification-related proteins. 16S rRNA sequencing performed delineate microbiota characteristics gut blood CKD-afflicted rats. Additionally, transcriptome tissue conducted explore relationship between CKD-associated features alterations function. Results: inhibited body weight gain, led pronounced aorta. both these affected rats exhibited significantly lower alpha diversity distinctive beta than those their healthy counterparts. resulted several indices (including elevated levels creatinine, low-density lipoprotein-cholesterol, sodium, phosphorous, total cholesterol, urea decreased albumin, calcium, lactate dehydrogenase, bilirubin). Moreover, it upregulated factors (bone sialoprotein [BSP], Klotho, fibroblast growth [FGF]-23, sclerostin [SOST]) lipopolysaccharide (LPS). Notably, increased Acinetobacter positively associated calcifications addition positive correlation microbiota. enrichment increases (BSP, FGF-23, SOST), LPS, phosphorous. Furthermore, revealed that correlated majority genes negatively downregulated involved mineral absorption pathway. Conclusion: Our findings, first time, underscore dysbiosis symbiotic microbiota, blood, Particularly, emerges potential its accompanying VC.