Dl-3-n-butylphthalide attenuates cerebral ischemia/reperfusion injury in mice through AMPK-mediated mitochondrial fusion

• DOI: 10.3389/fphar.2024.1357953
• Publication Date: 2024-02-22T05:26:22Z
• Authors: Ting Zhu; Shanshan Dong; Na Qin; Rujuan Liu; Liuliu Shi; Qi Wan
• Keywords: MFN1; Stroke

Introduction: NBP is a compound isolated from celery seeds, which was approved by the National Medical Products Administration in 2002 for clinical treatment of ischemic stroke. However, brain ischemia/reperfusion (I/R) injury, related research on mitochondrial dynamics and its mechanism action still need to be further studied. The aim this study assess cerebral pathology stroke vivo , with specific focus molecular mechanisms how promotes fusion. Methods: Male C57BL/6 mice were utilized subjected middle artery occlusion/reperfusion (MCAO/R). Pre-ischemia, administered through intraperitoneal (i.p.) injection 7 days. Results: Our findings demonstrated that effectively reduced infarct volume, improved neurological dysfunction, enhanced blood flow, promoted fusion MCAO/R. More importantly, pro-fusion effects found linked activation AMPK/Mfn1 pathway, function, partially eliminated inhibitors AMPK. Discussion: results revealed novel promoter protecting against AMPK-mediated Mfn1. These contribute understanding involved protection function following