Blood-supplementing effect of low molecular weight peptides of E-Jiao on chemotherapy-induced myelosuppression: evaluation of pharmacological activity and identification of bioactive peptides released in vivo

• DOI: 10.3389/fphar.2024.1366407
• Publication Date: 2024-06-05T04:40:49Z
• Authors: Jinju Zhang; Danlin Lin; Yuting Wu; Lixia Chen; Zhiguo Ma; Menghua Wu; Xindan Liu; Ying Zhang; Hui Cao
• Keywords: Equidae

Background: Equus asinus L. [Equidae; Asini Corri Colla] (donkey-hide gelatin, E-Jiao) is a traditional Chinese medicine renowned for its exceptional blood-supplementing effect. However, the specific components that contribute to efficacy remain elusive. This study aimed demonstrate peptides are responsible E-Jiao’s effect and explore contributing efficacy. Methods: The low molecular weight of E-Jiao (LMEJ) were obtained using an in vitro digestion method. LMEJ rat bloodstream characterized by peptidomics analysis. was assessed blood-deficient zebrafish mouse models. detected blood evaluated same model, network pharmacology analysis performed investigate underlying mechanisms. Results: A total 660 unique identified within LMEJ. Both significantly alleviated myelosuppression mice but only attenuated zebrafish. After administration rats, 67 E-Jiao-derived bloodstream, 41 which identical those Out these peptides, five synthesized. Subsequent verification their effects revealed two them able alleviate Network suggested may exert regulating signaling pathways such as JAK-STAT, IL-17 others. These results indicated at least partially Conclusion: provides crucial foundation further exploration bioactive E-Jiao.