Transcriptomics Analysis and Re-sequencing Reveal the Mechanism Underlying the Thermotolerance of an Artificial Selection Population of the Pacific Oyster

• DOI: 10.3389/fphys.2021.663023
• Publication Date: 2021-04-22T06:18:50Z
• Authors: Yulong Tan; Rihao Cong; Haigang Qi; Luping Wang; Guofan Zhang; Ying Pan; Li Li
• Keywords: Pacific oyster; Selective breeding; Candidate gene

The Pacific oyster is a globally important aquaculture species inhabiting the intertidal environment, which experiences great temperature variation. Mass deaths in summer pose major challenge for industry. We initiated an artificial selection breeding program 2017 using acute heat shock treatments of parents to select thermotolerance oysters. In this study, we compared respiration rate, survival gene expression, and structure F 2 selected oysters non-selected wild A transcriptional analysis revealed global divergence between control groups at larval stage, including 4764 differentially expressed genes, among 79 genes were heat-responsive genes. Five proteins enriched, four six (five stock enriched GO terms KEGG pathways BAG4 ) 1-year-old Integration transcriptomic re-sequencing data 1090 that differentiated both expression. Two SNPs (single nucleotide polymorphism) may mediate expression CGI_10022585 CGI_10024709 validated. addition, rate varied significantly group room (20°C). And population was improved. This study not only shows has significant effect on oysters, but it also helps reveal mechanism underlying their tolerance high as well ability adapt climate change.