Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry

• DOI: 10.3389/fphys.2021.761411
• Publication Date: 2021-10-21T06:54:32Z
• Authors: Lourdes Méndez-Mora; Maria Cabello-Fus...; Josep Ferré-Torres; Carla Riera-Llobet; Elena Krishnevskaya; Claudia Trejo-Soto; Salvador Payán-Pe...; Inés Hernández-Ro...; Cristian Morales-...; Tomas Alarcón; Joan-Lluis Vives-...; Aurora Hernandez-...
• Keywords: Hemorheology; Erythrocyte deformability

The purpose of this work is to develop a hematocrit-independent method for the detection beta-thalassemia trait (β-TT) and iron deficiency anemia (IDA), through rheological characterization whole blood samples from different donors. results obtained herein are basis development front microrheometry point-of-care device diagnosis clinical follow-up β-TT patients suffering hematological diseases alterations in morphology red cell (RBC). viscosity calculated as function mean velocity by detecting sample fluid-air interface advancing microfluidic channel. Different curves healthy donors, IDA samples. A mathematical model introduced compare distinct hematocrit, classifying curve patterns with respect health condition blood. fluid at certain shear rate values varies depending on several RBC factors such shape size, hemoglobin (Hb) content, membrane rigidity hematocrit concentration. Blood plasma donors used reference. To validate their potential value diagnostic tool, compared those gold-standard deformability evaluation, Laser-Optical Rotational Red Cell Analyzer (LoRRCA).