Evaluation of the transverse aortic constriction model in ICR and C57BL/6J mice

• DOI: 10.3389/fphys.2022.1026884
• Publication Date: 2022-11-29T08:44:04Z
• Authors: Mengying Huang; Lishuang Yu; Xiaoping Wang; Mingmin Wang; Weili Li; Jiayang Tang; Guanjing Ling; Xiaoqi Wei; Yong Wang; Wei Wang; Yan Wu; Linghui Lu
• Keywords: Pressure overload; Constriction; C57BL/6; Histology; Pulmonary artery banding

Transverse aortic constriction (TAC) is a frequently used model to investigate pressure overload-induced progressive heart failure (HF); however, there considerable phenotypic variation among different mouse strains and even sub-strains. Moreover, less known about the TAC in ICR mice. Therefore, determine suitability of strain for TAC-induced HF research, we compared effects on C57BL/6J mice at one, two four weeks post-TAC via echocardiography, organ index, morphology, histology. At end study, behavior gene expression patterns were assessed, overall survival was monitored. Compared sham-operated mice, displayed hypertrophic phenotypes with significant increase ventricle wall thickness, weight ratio, cross-sectional area cardiomyocytes after 2-week exposure. In addition, developed reduced systolic function severe lung congestion 4 post-TAC, whereas did not. Besides, demonstrated comparable survival, similar alteration but severer fibrotic remodeling poor behavioral performance Our data that quite sensitive can be an ideal research tool mechanisms drug intervention HF.