Storage-Induced Micro-Erythrocytes Can Be Quantified and Sorted by Flow Cytometry

• DOI: 10.3389/fphys.2022.838138
• Publication Date: 2022-02-23T17:32:15Z
• Authors: Mickaël Marin; Sandy Peltier; Youcef Hadjou; Sonia Georgeault; Michaël Dussiot; Camille Roussel; Olivier Hermine; Philippe Roingeard; Pierre A. Buffet; Pascal Amireault
• Keywords: Echinocyte; Cytometry; Cell Sorting

Refrigerated storage of red cell concentrates before transfusion is associated with progressive alterations blood cells (RBC). Small RBC (type III echinocytes, sphero-echinocytes, and spherocytes) defined as storage-induced micro-erythrocytes (SME) appear during pretransfusion storage. SME accumulate variable intensity from donor to donor, are cleared rapidly after transfusion, their proportion correlates recovery. They can be objectively quantified using imaging flow cytometry (IFC). Quantifying would further facilitate a physiologically relevant quality control concentrates. stored in bank conditions were stained carboxyfluorescein succinimidyl ester (CFSE) dye incubated at 37°C. CFSE was assessed by morphology evaluated IFC. We observed the accumulation high subpopulation that accounted for 3.3 47.2% day 3 42 storage, respectively. IFC brightfield images showed this mostly contains while low type I II echinocytes discocytes. Similar numbers (based on projected surface area) intensity). scanning electron microscopy ≥95% pure subpopulations obtained cytometry-based sorting. now common fluorescent standard cytometer. The staining protocol enables specific sorting SME, useful tool characterize targeted premature clearance transfusion.