Epigenetic Regulation of TET1-SP1 During Spermatogonia Self-Renewal and Proliferation

• DOI: 10.3389/fphys.2022.843825
• Publication Date: 2022-02-11T17:36:53Z
• Authors: Lingling Liu; Jin Wang; Shenghua Wang; Mudi Wang; Yuanhua Chen; Liming Zheng
• Keywords: 0303 health sciences; 03 medical and health sciences; Physiology; epigenetic modification; QP1-981; self-renewal; TET1; SP1; spermatogonia

Spermatogonia are the source of spermatogenic waves. Abnormal spermatogonia can cause ab-normal waves, which manifest as disorders such oligospermia, hypospermia, and azoospermia. Among them, self-renewal serves basis for maintaining process spermatogenesis, closely regulated balance between differentiation maintain continuous production spermatozoa. Tet methylcytosine dioxygenase 1(TET1) is an important epitope modifying enzyme that catalyzes conversion 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC), thereby causing methylation specific genes site hydroxylation, enabling DNA de-methylation process, regulating gene expression. However, hydroxymethylation sites at TET1 acts specifically mechanisms interaction affecting key differential not clear. In present study, we provide evidence expression PLZF, a marker self-renewal, was significantly elevated in overexpression group, while PCNA, proliferation-related gene, also mRNA level. Significant SP1 found by sequencing. increased both level protein after overexpression, DAXX downregulated level, its reciprocal P53 upregulated. conclusion, our results suggest causes changes SP1, other genes, there certain antagonistic effect DAXX, eventually reaches dynamic state sustained sperm production. These findings may contribute understanding male reproductive system disorders.