Genome-Wide Characterization of the C-repeat Binding Factor (CBF) Gene Family Involved in the Response to Abiotic Stresses in Tea Plant (Camellia sinensis)

• DOI: 10.3389/fpls.2020.00921
• Publication Date: 2020-07-24T09:53:03Z
• Authors: Zhe Hu; Qiuyan Ban; Jing Hao; Xiangxiang Zhu; Yaohua Cheng; Junlin Mao; Mengling Lin; Enhua Xia; Yeyun Li
• Keywords: 0301 basic medicine; 03 medical and health sciences; CBF gene family; transgenic Arabidopsis thaliana; cold stress; Plant culture; Plant Science; functional genomics; Camellia sinensis; SB1-1110

C-repeat (CRT)/dehydration responsive element (DRE)-binding factor CBFs, a small family of genes encoding transcriptional activators, play important roles in plant cold tolerance. In this study, comprehensive genome-wide analysis was carried out to identify and characterize the functional dynamics CsCBFs tea (Camellia sinensis). A total 6 CBF were obtained from genome named CBF1-6. All had an AP2/ERF DNA-binding domain nuclear localization signal (NLS) sequence. CsCBF-eGFP fusion DAPI staining confirmed CsCBFs. Transactivation assays showed that CsCBFs, except CsCBF1, activity. CsCBF expression differentially induced by cold, heat, PEG, salinity, ABA, GA, MeJA, SA stresses. particular, significantly treatments. To further functions genes, we overexpressed CsCBF3 gene Arabidopsis thaliana plants. The resulting transgenic plants increased tolerance compared with wild-type plant. enhanced potentially achieved through ABA-independent pathway. This study will help increase our understanding their contributions stress