The CLV3 Homolog in Setaria viridis Selectively Controls Inflorescence Meristem Size

• DOI: 10.3389/fpls.2021.636749
• Publication Date: 2021-02-15T08:11:28Z
• Authors: Chuanmei Zhu; Lei Liu; Olivia Crowell; Hui Zhao; Thomas P. Brutnell; David Jackson; Elizabeth A. Kellogg
• Keywords: 0301 basic medicine; Setaria; grass; FON2; Meristem; Arabidopsis; Plant Science; maize; meristem; Gene; SB1-1110; Molecular Mechanisms of Plant Development and Regulation; Molecular Mechanisms of Photosynthesis and Photoprotection; Agricultural and Biological Sciences; 03 medical and health sciences; Biochemistry, Genetics and Molecular Biology; CLV3/ESR-related; Genetics; Inflorescence; Molecular Biology; Biology; 580; 2. Zero hunger; Fertilization Mechanisms; Botany; Mutant; Plant culture; Life Sciences; Oryza; Panicle; Weed; Molecular Mechanisms of Pollen Development and Function; FOS: Biological sciences; gene expression; CLE; Setaria viridis; gene regulation; Bract; inflorescence development

The CLAVATA pathway controls meristem size during inflorescence development in both eudicots and grasses, and is initiated by peptide ligands encoded byCLV3/ESR-related (CLE) genes. While CLV3 controls all shoot meristems inArabidopsis, evidence from cereal grasses indicates that different meristem types are regulated by different CLE peptides. The rice peptide FON2 primarily controls the size of the floral meristem, whereas the orthologous peptides CLE7 and CLE14 in maize have their most dramatic effects on inflorescence and branch meristems, hinting at diversification among CLE responses in the grasses.Setaria viridisis more closely related to maize than to rice, so can be used to test whether the maize CLE network can be generalized to all members of subfamily Panicoideae. We used CRISPR-Cas9 inS. viridisto knock out theSvFON2gene, the closest homolog toCLV3andFON2.Svfon2mutants developed larger inflorescence meristems, as in maize, but had normal floral meristems, unlikeOsfon2, suggesting a panicoid-specific CLE network. Vegetative traits such as plant height, tiller number and leaf number were not significantly different between mutant and wild type plants, but time to heading was shorter in the mutants.In situhybridization showed strong expression ofSvfon2in the inflorescence and branch meristems, consistent with the mutant phenotype. Using bioinformatic analysis, we predicted the co-expression network ofSvFON2and its signaling components, which included genes known to control inflorescence architecture in maize as well as genes of unknown function. The similarity between SvFON2 function in Setaria and maize suggests that its developmental specialization in inflorescence meristem control may be shared among panicoid grasses.