In situ Phenotyping of Grapevine Root System Architecture by 2D or 3D Imaging: Advantages and Limits of Three Cultivation Methods

• DOI: 10.3389/fpls.2021.638688
• Publication Date: 2021-06-29T05:51:45Z
• Authors: Yuko Krzyzaniak; Frédéric Cointault; Camille Loupiac; Eric Bernaud; Frédéric Ott; Christophe Salon; Anthony Laybros; Simeng Han; Marie-Claire Héloir; Marielle Adrian; Sophie Trouvelot
• Keywords: Hydroponics; Root system; Cutting; Root (linguistics)

The root system plays an essential role in the development and physiology of plant, as well its response to various stresses. However, it is often insufficiently studied, mainly because difficult visualize. For grapevine, a plant major economic interest, there growing need study system, particular assess resistance biotic abiotic stresses, understand decline that may affect it, identify new ecofriendly production systems. In this context, we have evaluated compared three distinct methods (hydroponics, plane, cylindric rhizotrons) order describe relevant architectural traits grapevine cuttings (mode propagation), also two 2D- (hydroponics rhizotron) one 3D- (neutron tomography) imaging techniques for visualization quantification roots. We observed hydroponics tubes are easy implement but do not allow direct over time, conversely 2D rhizotron. demonstrated neutron tomography quantify volume. produced automated analysis method digital photographs, adapted identifying adventitious roots feature architecture rhizotrons. This integrates image segmentation, skeletonization, detection skeleton, reconstruction. Although was targeted most results obtained could be extended other plants propagated by cuttings. Image characterization from seedlings.