PIP2, An Auxin Induced Plant Peptide Hormone Regulates Root and Hypocotyl Elongation in Arabidopsis

• DOI: 10.3389/fpls.2021.646736
• Publication Date: 2021-05-14T10:28:15Z
• Authors: Saddam Hussain; Wei Wang; Sajjad Ahmed; Xutong Wang; Adnan; Yuxin Cheng; Chen Wang; Yating Wang; Na Zhang; Hainan Tian; Siyu Chen; Xiaojun Hu; Tianya Wang; Shucai Wang
• Keywords: Plant hormone; Lateral root

Auxin is one of the traditional plant hormones, whereas peptide hormones are peptides with hormone activities. Both auxin and regulate multiple aspects growth development, there cross-talks between hormones. PAMP-INDUCED SECRETED PEPTIDES (PIPs) PIP-LIKEs (PIPLs) a new family hormone, PIPL3/TARGET OF LBD SIXTEEN 2 (TOLS2) has been shown to lateral root formation in Arabidopsis . We report here identification PIP2 as an response gene, we found it plays role regulating hypocotyl development By using quantitative RT-PCR, that expression but not PIP1 PIP3 was induced by auxin, reduced nph4-1 arf19-4 , lost-of-function mutants Response Factor 7 ( ARF7 ) ARF19 respectively. generating characterizing overexpressing transgenic lines gene edited for length overexpression seedlings slightly shorter when compared Col wild type plants, pip2 mutant remained largely unchanged. For comparison, also generated well pip3 double mutants. Surprisingly, than seedlings, produced short roots. However, similar single seedlings. On other hand, elongation assays indicate only 35S:PIP2 longer hypocotyls Further analysis indicates promotes cell division hypocotyls. Taken together, our results suggest likely via elongation.