Characterization of Metal Tolerance Proteins and Functional Analysis of GmMTP8.1 Involved in Manganese Tolerance in Soybean

• DOI: 10.3389/fpls.2021.683813
• Publication Date: 2021-12-01T12:08:05Z
• Authors: Jifu Li; Rongshu Dong; Yidan Jia; Jie Huang; Xiaoyan Zou; Na An; Jianling Song; Zhijian Chen
• Keywords: 2. Zero hunger; 0301 basic medicine; 03 medical and health sciences; metal tolerance protein; Glycine max; cation diffusion facilitator; Mn detoxification; Plant culture; Mn toxicity; Plant Science; Mn transporter; SB1-1110

Manganese is an essential micronutrient for plant growth but can be toxic to plants when it reaches excessive levels. Although metal tolerance proteins (MTPs), which belong the cation diffusion facilitator (CDF) family, have been demonstrated play critical roles in manganese (Mn) plants, characteristics and functions of GmMTP members response soybean (Glycine max) Mn toxicity not documented. In this study, inhibition was observed that were exposed a level hydroponics, as reflected by generation brown spots, decreased leaf chlorophyll concentration fresh weight. Subsequent genome-wide analysis resulted identification total 14 genes genome. Among these GmMTPs, 9 12 found regulated excess leaves roots, respectively. Furthermore, function GmMTP8.1, Mn-CDF homologue ShMTP8 identified legume Stylosanthes hamata involved detoxification, characterized. Subcellular localization showed GmMTP8.1 localized endoplasmic reticulum (ER). Heterologous expression led restoration Mn-hypersensitive yeast (Saccharomyces cerevisiae) mutant Δpmr1, made defective transport into Golgi apparatus P-type Ca/Mn-ATPase. overexpression conferred Arabidopsis (Arabidopsis thaliana). Under conditions, concentrations shoots roots transgenic Arabidopsis, overexpressing compared wild type. The also upregulation several transporter responsible efflux sequestration such AtMTP8/11. Taken together, results suggest ER-localized contributing confer stimulating export out cells increasing intracellular compartments.