Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses

• DOI: 10.3389/fpls.2021.687799
• Publication Date: 2021-06-18T07:08:07Z
• Authors: Lalida Sangpong; Gholamreza Khaksar; Pinnapat Pinsorn; Akira Oikawa; Ryosuke Sasaki; Alexander Erban; Mutsumi Watanabe; Karan Wangpaiboon; Takayuki Tohge; Joachim Kopka; Rainer Hoefgen; Kazuki Saito; Supaart Sirikanta...
• Keywords: Metabolic pathway; Isoleucine; Carbohydrate Metabolism

Durian is an economically important fruit of Southeast Asia. There is, however, a lack in-depth information on the alteration its metabolic networks during ripening. Here, we annotated 94 ripening-associated metabolites from pulp durian cv. Monthong at unripe and ripe stages, using capillary electrophoresis- gas chromatography- time-of-flight mass spectrometry, specifically focusing taste-related metabolites. During ripening, sucrose content increased. Change in raffinose-family oligosaccharides are reported herein for first time. The malate succinate contents increased, while those citrate, abundant organic acid, were unchanged. Notably, most amino acids including isoleucine, leucine, valine, whereas aspartate decreased, glutamate was Furthermore, transcriptomic analysis performed to analyze dynamic changes sugar metabolism, glycolysis, TCA cycle, acid pathways identify key candidate genes. Taken together, our results elucidate fundamental metabolism durian, which can be exploited develop genetic markers future.