TaWRKY13-A Serves as a Mediator of Jasmonic Acid-Related Leaf Senescence by Modulating Jasmonic Acid Biosynthesis

• DOI: 10.3389/fpls.2021.717233
• Publication Date: 2021-09-04T01:45:08Z
• Authors: Hualiang Qiao; Yongwei Liu; Lingling Cheng; Xuelin Gu; Pengcheng Yin; Ke Li; Shuo Zhou; Geng Wang; Chunjiang Zhou
• Keywords: Jasmonic acid; Senescence; WRKY protein domain; Brachypodium distachyon; Brachypodium

Leaf senescence is crucial for crop yield and quality. Transcriptional regulation a key step integrating various senescence-related signals into the nucleus. However, few regulators of implicating transcriptional events have been functionally characterized in wheat. Based on our RNA-seq data, we identified WRKY transcription factor, TaWRKY13-A, that predominately expresses at senescent stages. By using virus-induced gene silencing (VIGS) method, manifested impaired TaWRKY13-A leading to delayed leaf phenotype Moreover, overexpression ( OE ) accelerated onset under both natural growth condition darkness Brachypodium distachyon Arabidopsis thaliana . Furthermore, by physiological molecular investigations, verified participates via jasmonic acid (JA) pathway. The expression JA biosynthetic genes, including AtLOX6 , was altered -overexpressing We also demonstrated can interact with promoter TaLOX6 electrophoretic mobility shift assay (EMSA) luciferase reporter system. Consistently, detected higher level lines than Col-0. data suggested partially functional conserved AtWRKY53 age-dependent senescence. Collectively, this study manifests as positive regulator JA-related senescence, which could be new clue breeding