Multi-Omics-Based Identification and Functional Characterization of Gh_A06G1257 Proves Its Potential Role in Drought Stress Tolerance in Gossypium hirsutum

• DOI: 10.3389/fpls.2021.746771
• Publication Date: 2021-10-21T16:27:19Z
• Authors: Teame Gereziher M...; Yanchao Xu; Muhammad Jawad Umer; Margaret Linyerer...; Yuqing Hou; Yuhong Wang; Shuxun Yu; Xianlong Zhang; Kunbo Wang; Xiaoyan Cai; Zhongli Zhou; Fang Liu
• Keywords: Drought Tolerance; Candidate gene

Cotton is one of the most important fiber crops globally. Despite this, various abiotic stresses, including drought, cause yield losses. We used transcriptome profiles to investigate co-expression patterns gene networks associated with drought stress tolerance. identified three modules containing 3,567 genes highly Within these modules, we 13 hub based on intramodular significance, for further validation. The yellow module has five (Gh_A07G0563, Gh_D05G0221, Gh_A05G3716, Gh_D12G1438, and Gh_D05G0697), brown contains belonging aldehyde dehydrogenase (ALDH) family (Gh_A06G1257, Gh_A06G1256, Gh_D06G1578), pink (Gh_A02G1616, Gh_D12G2599, Gh_D07G2232, Gh_A02G0527, Gh_D07G0629). Based RT-qPCR results, Gh_A06G1257 highest expression under in different plant tissues it might be true candidate linked tolerance cotton. Silencing cotton leaves conferred significant sensitivity response treatments. Overexpression Arabidopsis also confirms its role L-valine, Glutaric acid, L-proline, L-Glutamic L-Tryptophan were found metabolites playing roles These findings add significantly existing knowledge mechanisms