Transcriptomics and Metabolomics Changes Triggered by Inflorescence Removal in Panax notoginseng (Burk.)

• DOI: 10.3389/fpls.2021.761821
• Publication Date: 2021-11-12T17:12:40Z
• Authors: Yu Bai; Haijiao Liu; Jianying Pan; Shiyan Zhang; Yixin Guo; Yisha Xian; Zhirong Sun; Zilong Zhang
• Keywords: Panax notoginseng; WRKY protein domain; MYB

The root of Panax notoginseng (Burk.), in which saponins are the major active components, is a famous traditional Chinese medicine used to stop bleeding and decrease inflammation heart disease. Inflorescence removal increases yield quality P. notoginseng, but underlying molecular mechanisms unknown. Here, differences between inflorescence-removal treatment control groups were compared using transcriptomics metabolomics analyses. Illumina sequencing cDNA libraries prepared from rhizomes, leaves roots two independently identified 6,464, 4,584, 7,220 differentially expressed genes (DEG), respectively. In total, 345 transcription factors (TFs), including MYB WRKY family members, induced by treatment. Additionally, 215 DEGs involved saponin terpenoid backbone biosynthetic pathways identified. Most mevalonic acid (MVA) methylerythritol phosphate (MEP) activated inflorescence removal. co-expression analysis showed that low expression levels flavonoid biosynthesis-related (e.g., C4H F3H) decreased biosynthesis accumulation some flavonoids after results not only provide new insights into fundamental poorly studied process other rhizome crops, they also represent an important resource for future research on gene functions during treatments reproductive stage.