TaMYB29: A Novel R2R3-MYB Transcription Factor Involved in Wheat Defense Against Stripe Rust

• DOI: 10.3389/fpls.2021.783388
• Publication Date: 2021-12-01T13:07:35Z
• Authors: Xiaoxu Zhu; Xiang Li; Qi He; Dongxiao Guo; Caiqi Liu; Junying Cao; Zhongyi Wu; Zhensheng Kang; Xiaojing Wang
• Keywords: Jasmonic acid; MYB; Hypersensitive response

Members of the R2R3-MYB transcription factor superfamily have been implicated in plant development, improved disease resistance, and defense responses to several types stresses. To study function TaMYB29 factor-a member superfamily-in response an avirulent race stripe rust pathogen, Puccinia striiformis f. sp. tritici (Pst), we identified cloned gene from wheat cultivar (cv.) AvS+Yr10 following infection with Pst. The protein, comprising 261 amino acids, contains two highly conserved MYB domains. We first showed that is a factor, whose transcriptional levels are significantly induced by salicylic acid (SA), abscisic (ABA), jasmonic (JA), ethylene (ET), results involved stipe rust. overexpression resulted accumulation reactive oxygen species (ROS) pathogen-independent cell death Nicotiana benthamiana leaves. silencing cv. AvS+Yr10, containing resistance Yr10, promoted hyphae growth, downregulated expression pathogenesis-related (PR) genes, substantially reduced Pst compared non-silenced control. In addition, hydrogen peroxide (H2O2) decreased, activity catalase, enzyme required for H2O2 scavenging, was elevated. Altogether, positively regulates against enhancing accumulation, PR expression, SA signaling pathway-induced death. These provide new insights into contribution pathogens wheat.