Integrated Metabolomic and Transcriptomic Analysis of the Flavonoid Accumulation in the Leaves of Cyclocarya paliurus at Different Altitudes

• DOI: 10.3389/fpls.2021.794137
• Publication Date: 2022-02-08T05:25:23Z
• Authors: Zhaokui Du; Weida Lin; Binbin Yu; Jinxing Zhu; Junmin Li
• Keywords: Flavonoid Biosynthesis

Cyclocarya paliurus is a medicinal plant containing flavonoids, triterpenoids, polyphenolics, polysaccharides, and other compounds with diverse biological functions. C. distributed across altitudes ranging from 400 to 1,000 m. However, little known about the effect of altitude on metabolite accumulation in paliurus. Also, biosynthetic pathway involved flavonoid has not been fully elucidated. In this study, mature leaves growing at low (280 m) high (920 were sampled subjected metabolomic transcriptomic analyses. The content composition higher collected than those altitude. Most differentially accumulated metabolites (DAMs) enriched "flavone flavonol biosynthesis." significant expressed genes (DEGs) between mainly "biological process." most heavily KEGG was related subcategory "Oxidative phosphorylation," indicating that complicated processes are response harsh environmental factors. High UV-light might be main influencing factor among factors found altitudes. Integrated analysis data showed 31 flavonoids significantly correlated 227 DEGs, resulting 412 pairs (283 positive 129 negative) DEGs flavonoids. possible mechanisms underlying different due variations transport relocation leaves, but biosynthesis pathways. up-regulation energy protein synthesis contribute This study broadens our understanding