A Pathogen-Inducible Rice NAC Transcription Factor ONAC096 Contributes to Immunity Against Magnaprothe oryzae and Xanthomonas oryzae pv. oryzae by Direct Binding to the Promoters of OsRap2.6, OsWRKY62, and OsPAL1

• DOI: 10.3389/fpls.2021.802758
• Publication Date: 2021-12-10T06:45:30Z
• Authors: Hui Wang; Yan Bi; Yizhou Gao; Yuqing Yan; Xi Yuan; Xiaohui Xiong; Jiajing Wang; Jiayu Liang; Dayong Li; Fengming Song
• Keywords: Mechanisms of Plant Immune Response; 0301 basic medicine; Cell biology; OsPAL1; Xanthomonas oryzae pv. oryzae; Plant Science; Xanthomonas oryzae; Gene; SB1-1110; Agricultural and Biological Sciences; 03 medical and health sciences; Biochemistry, Genetics and Molecular Biology; Nicotiana benthamiana; Genetics; Molecular Biology; Biology; Elicitor Signal Transduction for Metabolite Production; 2. Zero hunger; Molecular Mechanisms of Flavonoid Biosynthesis in Plants; NAC; OsWRKY62; ONAC096; Plant culture; Life Sciences; Promoter; OsRap2.6; FOS: Biological sciences; Gene expression; Transcription factor; rice immunity; Transcription Factors

The rice NAC transcriptional factor family harbors 151 members, and some of them play important roles in immunity. Here, we report the function molecular mechanism a pathogen-inducible transcription factor, ONAC096, immunity against Magnaprothe oryzae Xanthomonas pv. . Expression ONAC096 was induced by M. abscisic acid methyl jasmonate. had DNA binding ability to recognition sequence found be nucleus-localized activator whose activity depended on its C-terminal. CRISPR/Cas9-mediated knockout attenuated X. as well suppressed chitin- flg22-induced reactive oxygen species burst expression PTI marker genes OsWRKY45 OsPAL4 ; contrast, overexpression enhanced these two pathogens strengthened or PTI. RNA-seq transcriptomic profiling qRT-PCR analysis identified small set defense signaling that are putatively regulated further biochemical validated could directly bind promoters OsRap2.6 , OsWRKY62 OsPAL1 three known regulate interacts with ONAC066, which is positive regulator These results demonstrate positively contributes through direct downstream target including