Sprayed biodegradable liquid film improved the freezing tolerance of cv. Cabernet Sauvignon by up-regulating soluble protein and carbohydrate levels and alleviating oxidative damage

• DOI: 10.3389/fpls.2022.1021483
• Publication Date: 2022-11-01T05:18:25Z
• Authors: Xing Han; Fei Yao; Ting-ting Xue; Zhi-lei Wang; Ying Wang; Xiao Cao; Miao Hui; Dong Wu; Yi-han Li; Hua Wang; Hua Li
• Keywords: Overwintering; Malondialdehyde

Most cultivars of Vitis vinifera L. are very sensitive to cold. As an exogenous protectant, Biodegradable Liquid Film (BLF) is considered protect winegrapes from low temperatures and dry winds for safe overwintering. This study aimed reveal the physiological biochemical mechanisms BLF regulating freezing tolerance wine grapes. Groups ten-year-old vines (Cabernet Sauvignon) were sprayed with in November 2020 2021, or left untreated as a control treatment, field plant mortality after overwintering investigated. Branch samples collected monthly determination indicators. Dormant two-year-old cuttings also used relative expression levels key genes. The results showed that application reduced branch semi-lethal temperature January February compared control, above-ground parts, branches buds. status shoots was greatly affected by climatic conditions year, but treatment increased soluble protein sugar, decreased content superoxide anion malondialdehyde at most sampling times. Correlation analysis differences between no treated overwintering(CK) mainly related peroxidase activity, reducing sugar starch content. Low stress activated over ICE1, CBF1, CBF3, especially 12h. significantly CBF1 CBF3 under stress. Overall, these demonstrate protects damage upregulating osmo-regulatory substances alleviating oxidative damage.