Genetic diversity and structure of Bipolaris oryzae and Exserohilum rostratum populations causing brown spot of rice in Burkina Faso based on genotyping-by-sequencing

• DOI: 10.3389/fpls.2022.1022348
• Publication Date: 2022-11-25T05:53:12Z
• Authors: Kouka Hilaire Kaboré; Abalo Itolou Kass...; Henri Adreit; Joëlle Milazzo; Sonia Guillou; Laurence Blondin; Laurie Chopin; Sébastien Ravel; Florian Charriat; Mariam Barro; Charlottte Tollen...; Marc-Henri Lebrun; Didier Tharreau
• Keywords: Nucleotide diversity

In recent years, Brown spot disease of rice (BSR) has been observed on leaves and seeds in all rice-growing areas Burkina Faso. Bipolaris oryzae Exserohilum rostratum are the main fungal species isolated from BSR infected tissues they frequently same field. However, we lacking information genetic diversity population structure these fungi The mode reproduction is also unknown. isolates B. (n=61) E. (n=151), collected major Faso, was estimated using genotyping-by-sequencing (GBS). mean values for nucleotide (π) were 1.9 x10-4 4.8 rostratum. There no differentiation between geographical populations each species. analysis molecular variance revealed that 89% 94% variances within rostratum, respectively. For species, four clusters identified by two clustering methods (DAPC sNMF). distribution groups independent origin isolates. Evidence recombination detected balanced mating type ratios supporting sexual reproduction. overrepresentation MAT1-2 (79%) suggested a predominant asexual This study provides important biology genetics causing brown