Experimental rice seed aging under elevated oxygen pressure: Methodology and mechanism

• DOI: 10.3389/fpls.2022.1050411
• Publication Date: 2022-12-01T17:58:18Z
• Authors: Manjunath Prasad ...; Jan Kodde; Gerco C. Angenent; Ric C. H. de Vos; Carmen Diez-Simon; Roland Mumm; Fiona R. Hay; Sasiwimon Siricha...; Devendra K. Yadava; Steven P. C. Groot
• Keywords: Accelerated aging; Senescence; Partial pressure

Seed aging during storage results in loss of vigor and germination ability due to the accumulation damage by oxidation reactions. Experimental tests, for instance study genetic variation, aim mimic natural a shorter timeframe. As rate is increased elevating temperature, moisture, oxygen levels, this aimed (1) investigate effect experimental rice seed an elevated partial pressure (EPPO), (2) elucidate mechanism dry-EPPO (3) compare under conditions traditional moist-controlled deterioration (CD) long-term ambient storage. Dry seeds from 20 diverse accessions were experimentally aged EPPO (200 times higher levels), at 50% relative humidity (RH), along with high-pressure nitrogen gas as controls. While no decline was observed storage, there significant considerable variation among accessions, average toward survival obtained after around 21 days total 56 days. Storage resulted small but decline, 5% In second experiment, seven lots stored compared moist-CD test two different conditions, i.e., conditioned warehouse (CWSS) (TRSS). Untargeted metabolomics (with identification lipid volatile compounds profiles) showed relatively high increase levels oxidized lipids related volatiles all four conditions. These had negative correlation viability, indicating main deteriorating process aging. Correlation analysis indicated that RH more TRSS 60% CD-aging 75% ERH rather than CWSS 40% ERH. conclusion, suitable method analyzing or genotypes longevity