Functional genomic analysis of K+ related salt-responsive transporters in tolerant and sensitive genotypes of rice

• DOI: 10.3389/fpls.2022.1089109
• Publication Date: 2023-01-19T12:21:06Z
• Authors: Umme Sabrina Haque; Sabrina M. Elias; Israt Jahan; Zeba I. Seraj
• Keywords: Inbred strain

Introduction Salinity is a complex environmental stress that affects the growth and production of rice worldwide. But there are some landraces in coastal regions can survive presence highly saline conditions. An understanding molecular attributes contributing to salinity tolerance these genotypes important for developing salt-tolerant high yielding modern ensure food security. Therefore, we investigated role functional differences two K + salt-responsive transporters. These OsTPKa or Vacuolar two-pore potassium channel OsHAK_like hypothetical protein HAK family. transporters were selected from previously identified QTLs tolerant landrace genotype (Horkuch) sensitive (IR29). Methods In silico comparative sequence analysis promoter sequences genes between Horkuch IR29 was done. Real-Time expression leaves roots (salt-sensitive), I-14 I-71 (Recombinant Inbred Lines IR29(♀)× Horkuch), Pokkali (salt-tolerant) under salt-stress at different time points analyzed. For further insight, chosen loss-of-function genomic using CRISPR/Cas9 tool. Furthermore, cloning into variety by Gateway technology observe effect gain-of-function. Results The showed significant which may give survival advantage salt-stress. also found be overexpressed varieties (Horkuch Pokkali). Moreover, coordinated pattern observed Independently transformed plants where significantly lowered, performed poorly physiological tests tolerance. On other hand, positively T 0 with gene consistently both control salt stress. Discussion poor performance transgenic down-regulated supports assumption play roles defending against stress, minimizing injury, maintaining plant growth. provided overexpression vacuolar transporter, particularly reconfirms its providing QTL locus containing maybe bred commercial produce high-yielding rice.