Application of chloroplast genome in the identification of Phyllanthus urinaria and its common adulterants

• DOI: 10.3389/fpls.2022.1099856
• Publication Date: 2023-01-06T17:29:13Z
• Authors: Hui Fang; Guona Dai; Binbin Liao; Ping Zhou; Yinglin Liu
• Keywords: Phyllanthus; Identification; Molecular marker

Background Phyllanthus urinaria L. is extensively used as ethnopharmacological material in China. In the local marketplace, this medicine can be accidentally contaminated, deliberately substituted, or mixed with other related species. The contaminants herbal products are a threat to consumer safety. Due scarcity of genetic information on plants, more molecular markers needed avoid misidentification. Methods study, complete chloroplast genome nine species genus was de novo assembled and characterized. Results This study revealed that all these exhibited conserved quadripartite structure, which includes large single copy (LSC) region small (SSC) region, two copies inverted repeat regions (IRa IRb), separate LSC SSC regions. And codon usage, sequences were highly showed similarities among Three variable ( trnS-GCU-trnG-UCC , trnT-UGU-trnL-UAA petA-psbJ ) might helpful potential for identifying P. its contaminants. addition, clock analysis results divergence time occur at ~ 48.72 Ma. Conclusion provides valuable further identification, evolution, phylogenetic research .