Effect of azoxystrobin on tobacco leaf microbial composition and diversity

• DOI: 10.3389/fpls.2022.1101039
• Publication Date: 2023-02-01T14:39:51Z
• Authors: Meili Sun; Hancheng Wang; Caihua Shi; Jianjun Li; Liuti Cai; Ligang Xiang; Tingting Liu; Paul H. Goodwin; Xingjiang Chen; Ling Wang
• Keywords: 2. Zero hunger; 0301 basic medicine; tobacco target spot; 03 medical and health sciences; microbial composition; leaf microorganisms; high-throughput sequencing; Plant culture; azoxystrobin; Plant Science; SB1-1110

Azoxystrobin, a quinone outside inhibitor fungicide, reduced tobacco target spot caused by Rhizoctonia solani 62%, but also affected the composition and diversity of other microbes on surface interior treated leaves. High-throughput sequencing showed that dominant bacteria prior to azoxystrobin treatment were Methylobacterium healthy leaves Pseudomonas diseased leaves, fungi Thanatephorous (teleomorph Rhizoctonia) Symmetrospora Both bacterial fungal significantly increased 1 18 days post (dpt) with for For relative abundance Pseudomonas, Sphingomonas, Unidentified-Rhizobiaceae Massilia declined, while Aureimonas increased. On Sphingomonas Methylobacterium, Pantoea Symmetrospora, Sampaiozyma, Plectosphaerella, Cladosporium Cercospora Cladosporium, Phoma, Pantospora Fusarium, Compared resulted in greater reductions Thanatephorous, Unidentified-Rhizobiaceae, increase similar changes Plectosphaerella Cladosporium. Azoxystrobin had semi-selective effect altering microbial leaf microbiome, which could be due factors, such as differences among species sensitivity inhibitors, ability use nutrients niches certain are affected, metabolic responses azoxystrobin.