Myco-Synergism Boosts Herbivory-Induced Maize Defense by Triggering Antioxidants and Phytohormone Signaling

• DOI: 10.3389/fpls.2022.790504
• Publication Date: 2022-02-17T10:51:29Z
• Authors: Raufa Batool; Muhammad Jawad Umer; Yangzhou Wang; Kanglai He; Muhammad Zeeshan ...; Tiantao Zhang; Shuxiong Bai; Jie Chen; Zhenying Wang
• Keywords: Jasmonic acid; Oxylipin; Jasmonate

Biocontrol strategies are the best possible and eco-friendly solution to develop resistance against O furnacalis improve maize yield. However, knowledge about underlying molecular mechanisms, metabolic shifts, hormonal signaling is limited.Here, we used an axenic a consortium of entomopathogenic Beauveria bassiana OFDH1-5 pathogen-antagonistic Trichoderma asperellum GDFS1009 in observed that applications resulted higher chlorophyll contents antioxidants activities [superoxide dismutase (SOD), peroxidase (POD), proline, protease, polyphenol oxidase (PPO)] with decrease O. survival. We performed comprehensive transcriptome untargeted metabolome profiling for first time at vegetative stage fungal inoculated leaves 0-, 12-, 24-, 48-, 72-h post insect infestation.The B. T. leads 80-95% mortality. A total 13,156 differentially expressed genes were weighted gene coexpression network analysis. identified six significant modules containing thirteen candidate [protein kinase (GRMZM2G025459), acyl-CoA dehydrogenase (GRMZM5G864319), thioredoxin (GRMZM2G091481), glutathione S-transferase (GRMZM2G116273), patatin-like phospholipase (GRMZM2G154523), cytochrome P450 (GRMZM2G139874), protease inhibitor (GRMZM2G004466), (AC233926.1_FG002), chitinase (GRMZM2G453805), defensin (GRMZM2G392863), (GRMZM2G144153), GDSL- like lipase (AC212068.4_FG005), Beta-glucosidase (GRMZM2G031660)], which not previously reported highly correlated Jasmonic acid - Ethylene (JA-ET) pathway antioxidants. detected 130 negative 491 positive metabolomic features using ultrahigh-performance liquid chromatography ion trap time-of-flight mass spectrometry (UHPLC-QTOF-MS). Intramodular significance real time-quantitative polymerase chain reaction (RT-qPCR) expressions showed these true genes. Consortium treated had jasmonic (JA), salicylic (SA), ethylene (ET) levels.Our results provide insights into genetics, biochemicals, diversity useful future biocontrol ACB attacks.