Effects of Litter and Root Manipulations on Soil Bacterial and Fungal Community Structure and Function in a Schrenk’s Spruce (Picea schrenkiana) Forest

• DOI: 10.3389/fpls.2022.849483
• Publication Date: 2022-04-14T13:06:08Z
• Authors: Haiqiang Zhu; Lu Gong; Yan Luo; Junhu Tang; Zhaolong Ding; Xiaochen Li
• Keywords: Acidobacteria; Litter; Plant litter; Soil microbiology; Ectomycorrhiza

Soil microorganisms are the key driver of geochemical cycle in forest ecosystem. Changes litter and roots can affect soil microbial activities nutrient cycling; however, impact this change on community composition function remain unclear. Here, we explored effects root manipulations [control (CK), doubled input (DL), removal (NL), exclusion (NR), a combination (NI)] bacterial fungal communities functional groups during 2-year field experiment, using illumina HiSeq sequencing coupled with prediction platform PICRUSt FUNGuild. Our results showed that decreased diversity bacteria fungi (AEC, Shannon, Chao1). The under different treatments were dominated by phyla Proteobacteria, Acidobacteria, Actinomycetes, NL NR reduced relative abundance first two phyla. For communities, Basidiomycetes, Ascomycota, Mortierellomycota dominant DL increased while Ascomycota. We also found altered related to metabolism cofactors vitamins, lipid metabolism, biosynthesis other secondary metabolites, environmental adaptation, cell growth, death. including ectomycorrhizal, ectomycorrhizal-orchid mycorrhizal root-associated biotrophs saprotrophs among treatments. organic carbon (SOC), pH, water content important factors driving changes respectively. demonstrate plant detritus structure affecting physicochemical factors, which provides data for understanding material ecosystems global change.