Genetic Mapping of the Gmpgl3 Mutant Reveals the Function of GmTic110a in Soybean Chloroplast Development

• DOI: 10.3389/fpls.2022.892077
• Publication Date: 2022-05-26T08:57:47Z
• Authors: Hui Yu; Qiushi Wang; Zhirui Zhang; Tao Wu; Xinjing Yang; Xiaobin Zhu; Yongheng Ye; Jiantian Leng; Suxin Yang; Xianzhong Feng
• Keywords: GmTic110a; GmTic20; 0301 basic medicine; 2. Zero hunger; 03 medical and health sciences; Gmpgl3 mutant; Plant culture; gene mapping; Plant Science; soybean; GmTic40a/b; SB1-1110

The generation of oxygen and organic matter in plants mainly depends on photosynthesis, which directly affects plant growth development. chloroplast is the main organelle photosynthesis occurs. In this study, a Glycine max pale green leaf 3-1 ( Gmpgl3-1 ) mutant was isolated from soybean mutagenized population. presented with decreased chlorophyll contents, reduced stroma thylakoids, yields, numbers pods per plant. Bulked segregant analysis (BSA) together map-based cloning revealed single-nucleotide non-synonymous mutation at 341st nucleotide first exon development-related GmTic110a gene. phenotype knockout same as that mutant. gene highly expressed leaves various developmental stages, its protein localized to inner membrane. Split luciferase complementation assays coimmunoprecipitation (co-IP) experiments interacted GmTic20, GmTic40a, GmTic40b tobacco leaves. These results indicated plays an important role