Functional Diversification Analysis of Soybean Malectin/Malectin-Like Domain-Containing Receptor-Like Kinases in Immunity by Transient Expression Assays

• DOI: 10.3389/fpls.2022.938876
• Publication Date: 2022-06-23T09:41:49Z
• Authors: Qian Zhang; Shuxian Chen; Yazhou Bao; Dongmei Wang; Weijie Wang; Rubin Chen; Yixin Li; Guangyuan Xu; Xianzhong Feng; Xiangxiu Liang; Daolong Dou
• Keywords: Plant Immunity

Plants have responded to microbial pathogens by evolving a two-tiered immune system, involving pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered (ETI). Malectin/malectin-like domain-containing receptor-like kinases (MRLKs) been reported participate in many biological functions plant including resistance. However, little is known regarding the role of MRLKs soybean immunity. This crucial question address because an important source oil proteins, its production threatened various pathogens. Here, we systematically identified 72 Glycine max (GmMRLKs) demonstrated that them are transcriptionally induced or suppressed response infection with Next, successfully cloned 60 GmMRLKs subsequently characterized their roles transiently expressing Nicotiana benthamiana , model widely used study host-pathogen interactions. Specifically, examined effect on PTI responses noticed number negatively regulated reactive oxygen species burst flg22 chitin, cell death triggered XEG1 INF1. We also analyzed effectors AvrB- XopQ-induced hypersensitivity several ETI activation. further showed regulate probably coupling receptor complexes. Furthermore, transient expression selected hairy roots conferred reduced resistance pathogen Phytophthora sojae. In summary, revealed common specific as candidate susceptible genes may be useful for improving