Drought-responsive WRKY transcription factor genes IgWRKY50 and IgWRKY32 from Iris germanica enhance drought resistance in transgenic Arabidopsis

• DOI: 10.3389/fpls.2022.983600
• Publication Date: 2022-09-06T09:57:21Z
• Authors: Jingwei Zhang; Dazhuang Huang; Xiaojie Zhao; Man Zhang; Qian Wang; Xueyan Hou; Dongliu Di; Beibei Su; Shaokun Wang; Pai Sun
• Keywords: WRKY protein domain; Drought Tolerance

Drought greatly affects the growth and development of garden plants their ornamental value. WRKY transcription factors make up one largest factor families in they play an important role plant response to drought stress. However, function gene stress Iris germanica, which is commonly used landscaping, has not been studied. In this study, we isolated two genes from IgWRKY50 IgWRKY32, belong Group II III family, respectively. IgWRKY32 could be induced by PEG-6000, high temperature ABA germanica. quickly respond peaked at 3 h after PEG-6000 treatment (19.93- 23.32-fold). The fusion proteins IgWRKY50-GFP IgWRKY32-GFP were located nucleus mesophyll protoplasts Arabidopsis. overexpression improved osmotic tolerance transgenic Arabidopsis, mainly exhibited having a higher germination rate longer total root length on 1/2 MS medium containing mannitol. Under stress, had stomatal closure than wild type (WT). natural water loss leaves Arabidopsis was lower that WT, contents proline (Pro) soluble protein (SP) activities superoxide dismutase (SOD), peroxidase (POD) catalase (CAT) higher, but content malondialdehyde (MDA) lower. Furthermore, expression several stress-related (RD29A, DREB2A, PP2CA, ABA2) significantly increased IgWRKY50- IgWRKY32- overexpressing Arabidposis treatment. These results suggest as positive regulators, enhance resistance mediating signal transduction pathway. can candidate for molecular breeding Iris.