Roles of type II H+-PPases and PPsPase1/PECP2 in early developmental stages and PPi homeostasis of Arabidopsis thaliana

• DOI: 10.3389/fpls.2023.1031426
• Publication Date: 2023-01-27T06:59:06Z
• Authors: Hiroshi Tojo; Hiromitsu Tabeta; Shizuka Gunji; Masami Y. Hirai; Pascale David; Hélène Javot; Ali Ferjani
• Keywords: Homeostasis

The regulation of intracellular pyrophosphate (PPi) level is crucial for proper morphogenesis across all taxonomic kingdoms. PPi released as a byproduct from ~200 metabolic reactions, then hydrolyzed by either membrane-bound (H+-PPase) or soluble pyrophosphatases (PPases). In Arabidopsis, the loss vacuolar H+-PPase/FUGU5, key enzyme in homeostasis, results delayed growth and number developmental defects, pointing to importance homeostasis plant morphogenesis. Arabidopsis genome encodes several PPases addition FUGU5, such PPsPase1/PECP2, VHP2;1 VHP2;2, although their significance regarding remains elusive. Here, assess contribution, phenotypic analyses cotyledon aspect ratio, palisade tissue cellular phenotypes, adaxial side pavement cell complexity, stomatal distribution, etiolated seedling length were performed, provided that they altered due excess fugu5 mutant background. Overall, our revealed above five traits unaffected ppspase1/pecp2, vhp2;1 vhp2;2 loss-of-function mutants, well lines constitutively overexpressing PPsPase1/PECP2. Furthermore, metabolomics seedlings exhibited profiles comparable wild type. Together, these indicate contribution VHP2;2 levels negligible comparison FUGU5 early stages development.