Effects of different concentrations of biochar amendments and Pb toxicity on rhizosphere soil characteristics and bacterial community of red clover (Trifolium pretense L.)

• DOI: 10.3389/fpls.2023.1112002
• Publication Date: 2023-03-28T12:46:10Z
• Authors: Lingdong Meng; Yuchen Wu; Meiqi Mu; Zicheng Wang; Zirui Chen; Lina Wang; Zewang Ma; Guowen Cui; Xiujie Yin
• Keywords: Red Clover

Amending soil with biochar can reduce the toxic effects of heavy metals (HM) on plants and soil. However, different concentrations properties microbial activities in lead (Pb)-contaminated soils are unclear. In this study, two Pb were set (low, 1000 mg/kg; high, 5000 mg/kg), five corn straw (CSB) (0, 2.5, 5, 10 15%) used to determine response growth rhizosphere red clover (Trifolium pretense L.) (in terms bacteria) CSB application. The results showed that 5% better alleviated toxicity shoot length clover, biomass increased by 74.55 197.76% respectively reduced enrichment factor (BCF) transport (TF) clover. nutrients, catalase (CAT), acid phosphatase (ACP) urease activity, while addition pH, organic matter (SOM) content enzyme activity. 16S rDNA amplicon sequencing analysis diversity bacteria relative abundance plant growth-promoting rhizobacteria such as Gemmatimonas, Devosia Bryobacter. Spearman correlation alkaline restored positively correlated Chitinophaga, Sphingomonas, Pseudomonas, thus environment. This study demonstrates alleviate We also provide a theoretical basis for subsequent use beneficial regulate repair efficiency