Molecular identification of phenylalanine ammonia lyase-encoding genes EfPALs and EfPAL2-interacting transcription factors in Euryale ferox

• DOI: 10.3389/fpls.2023.1114345
• Publication Date: 2023-03-17T12:25:57Z
• Authors: AiLian Liu; Yue Zhu; YuHao Wang; TianYu Wang; ShuPing Zhao; Kai Feng; LiangJun Li; Peng Wu
• Keywords: Flavonoid Biosynthesis; Phenylalanine ammonia-lyase

Flavonoids are one of the most important secondary metabolites in plants, and phenylalanine ammonia-lyase (PAL) is first rate-limiting enzyme for their biosynthesis. However, detailed information on regulation PAL plants still little. In this study, E. ferox was identified functionally analyzed, its upstream regulatory network investigated. Through genome-wide identification, we obtained 12 putative genes from ferox. Phylogenetic tree synteny analysis revealed that expanded mostly preserved. Subsequently, activity assays demonstrated EfPAL1 EfPAL2 both catalyzed production cinnamic acid only, with exhibiting a superior activity. Overexpression Arabidopsis thaliana, respectively, enhanced biosynthesis flavonoids. Furthermore, two transcription factors, EfZAT11 EfHY5, were by yeast one-hybrid library as binding to promoter EfPAL2, further luciferase (LUC) indicated promoted expression while EfHY5 repressed EfPAL2. These results suggested positively negatively regulate flavonoid biosynthesis, respectively. Subcellular localization localized nucleus. Our findings clarified key established which would provide novel study mechanism.