Insights into the impacts of autotoxic allelochemicals from rhizosphere of Atractylodes lancea on soil microenvironments

• DOI: 10.3389/fpls.2023.1136833
• Publication Date: 2023-03-10T04:42:25Z
• Authors: Meng Wang; Juan Deng; Gonghao Duan; Lei Chen; Xiao Huang; Wenjie Wang; Ling Gong; Yan Zhang; Kun Yu; Lanping Guo
• Keywords: Allelopathy; Acidobacteria

Atractylodes lancea suffers from continuous cropping obstacles that have become a major constraint in its cultivation, but there is still little information on the autotoxic allelochemicals and their interaction with soil microorganisms. In this study, we firstly identified rhizosphere of A. determined autotoxicity. Third-year soils, i.e., rhizospheric bulk soil, compared control one-year natural fallow were used to determine biochemical properties microbial community. Eight roots detected exhibited significant autotoxicity effects seed germination seedling growth highest content dibutyl phthalate lowest IC50 value 2,4-di-tert-butylphenol inhibiting germination. The contents nutrients organic matter, pH value, enzyme activity altered between different parameters close those unplanted soil. PCoA analysis indicated community composition both bacteria fungi differed significantly among samples. Continuous decreased OTUs numbers bacterial fungal communities, restored them. relative abundance Proteobacteria, Planctomycetes, Actinobacteria decreased, Acidobacteria Ascomycota increased after three years cultivation. LEfSe 115 49 biomarkers for respectively. results suggested structure Overall, our revealed caused variations microenvironments resulted replantation problem lancea, alleviated deterioration by remodeling restoring properties. These findings provide important insights clues solving problems guiding management sustainable farmland.