Comparative gut proteomics study revealing adaptive physiology of Eurasian spruce bark beetle, Ips typographus (Coleoptera: Scolytinae)

• DOI: 10.3389/fpls.2023.1157455
• Publication Date: 2023-11-23T15:51:53Z
• Authors: Muhammad Zubair A...; Kanakachari Mogil...; Gothandapani Sell...; Valentina Siino; Fredrik Levander; Amit Roy
• Keywords: Proteome; Metabolic pathway

The bark beetle, Ips typographus (L.), is a major pest of Norway spruce, Picea abies causing enormous economic losses globally. adult stage the I. has complex life cycle (callow and sclerotized); callow beetles feed ferociously, whereas sclerotized male are more aggressive pioneers in establishing new colonies. We conducted comparative proteomics study to understand female digestion detoxification processes beetles. Proteome profiling was performed using high-throughput liquid chromatography-mass spectrometry. A total >3000 proteins were identified from beetle gut, among them, 539 differentially abundant (fold change ±2, FDR <0.05) between (DAPs) mainly engage with binding, catalytic activity, anatomical hydrolase metabolic process, carbohydrate metabolism, hence may be crucial for growth, digestion, detoxification, signalling. validated selected DAPs RT-qPCR. Gut enzymes such as NADPH-cytochrome P450 reductase (CYC), glutathione S-transferase (GST), esterase (EST) play role digesting host allelochemicals. enzyme activity assays them observed positive correlation CYC GST activities proteomic results, EST not fully correlated. Furthermore, our investigation revealed that had an upregulation associated juvenile hormone (JH) biosynthesis chitin exhibited linked fatty acid metabolism TCA cycle. These distinctive patterns protein regulation functional specific each developmental stage, underscoring adaptive responses typographicus overcoming conifer defences facilitating their survival. Taken together, it first gut comparing males females typographus, shedding light on ecology at molecular level. information about handling nutritionally limiting defence-rich spruce phloem diet can utilized formulate RNAi-mediated management.