Genome-wide identification and expression analysis of TCP family genes in Catharanthus roseus

• DOI: 10.3389/fpls.2023.1161534
• Publication Date: 2023-04-12T04:34:50Z
• Authors: Juan Hao; Lijun Zheng; Yidie Han; Hongshan Zhang; Kailin Hou; Xueshuang Liang; Cheng Chen; Zhijing Wang; Jiayi Qian; Zhihao Lin; Zitong Wang; Houqing Zeng; Chenjia Shen
• Keywords: Catharanthus roseus; Identification

The anti-tumor vindoline and catharanthine alkaloids are naturally existed in Catharanthus roseus (C. roseus), an ornamental plant many tropical countries. Plant-specific TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors play important roles various developmental processes. However, the of C. TCPs (CrTCPs) terpenoid indole alkaloid (TIA) biosynthesis largely unknown. Here, a total 15 CrTCP genes were identified newly updated genome grouped into three major classes (P-type, C-type CYC/TB1). Gene structure protein motif analyses showed that CrTCPs have diverse intron-exon patterns distributions. A number stress responsive cis-elements promoter regions CrTCPs. Expression analysis (CrTCP2, CrTCP4, CrTCP7) expressed specifically leaves four (CrTCP13, CrTCP8, CrTCP6, CrTCP10) flowers. HPLC contents classic TIAs, vindoline, ajmalicine, significantly increased by ultraviolet-B (UV-B) methyl jasmonate (MeJA) leaves. By analyzing expression under UV-B radiation MeJA application with qRT-PCR, TIA biosynthesis-related to be treatments. Interestingly, two TCP binding elements (GGNCCCAC GTGGNCCC) several genes, suggesting they potential target These results suggest involved regulation provide basis for further functional identification