The rootstock shape microbial diversity and functionality in the rhizosphere of Vitis vinifera L. cultivar Falanghina

• DOI: 10.3389/fpls.2023.1205451
• Publication Date: 2023-08-15T00:49:14Z
• Authors: Daniela Zuzolo; Maria Antonietta ...; Maria Maisto; Maria Tartaglia; Antonello Prigion...; Alessandra Falzarano; Giuseppe Marotta; Rosaria Sciarrillo; Carmine Guarino
• Keywords: 0303 health sciences; 03 medical and health sciences; microbial diversity; microbial terroir; Plant culture; Plant Science; rootstock; rhizosphere; grapevine; holobiont; SB1-1110

The rhizosphere effect occurring at the root-soil interface has increasingly been shown to play a key role in plant fitness and soil functionality, influencing plants resilience. Here, for first time, we investigated whether rootstock genotype on which Vitis vinifera L. cultivar Falanghina is grafted can influence microbiome. Specifically, evaluated extent 5BB 1103P rootstocks are able shape microbial diversity of environment. Moreover, explored potential function community its shift under influence. We seven vineyards subjected same pedo-climatic conditions, similar age, training system management collected twelve samples metagenomic analyses composite physical-chemical properties. In this study, used 16S rRNA gene-based analysis investigate bacterial composition. Liner discriminant size (LEFSe) was conducted biomarker discovery. functional composition sampled communities determined using PICRUSt, based marker gene sequencing profiles. Soil involved determination texture, pH, Cation Exchange Capacity (CSC), Organic Carbon (OC), electrical conductivity (EC), calcium (Ca), magnesium (Mg), potassium (K) content, Phosphorous (P), nitrogen (N). latter revealed that features were quite homogenous. data showed alpha-diversity (Observed OTUs) significantly increased microbiota. Irrespective cultivar, Pseudomonadota dominant phylum, followed by Actinomycetota > Bacteroidota Thermoproteota. However, major phyla differentiating associated with different types. At genus level, several taxa belonging Alphaproteobacteria classes enriched rhizosphere. Investigating profile, found most enzyme-encoding genes N cycling more abundant soil. C cycle Plant Growth Promotion (PGP) functionality. Our results suggest not only recruit specific communities, but also traits within