Genome-wide identification and expression analysis of the GRAS family under low-temperature stress in bananas

• DOI: 10.3389/fpls.2023.1216070
• Publication Date: 2023-08-31T20:12:00Z
• Authors: Ning Tong; Dan Li; Shuting Zhang; Mengjie Tang; Yukun Chen; Zihao Zhang; Yuji Huang; Yuling Lin; Zhenguang Cheng; Zhongxiong Lai
• Keywords: Identification

GRAS, named after GAI, RGA, and SCR, is a class of plant-specific transcription factors family that plays crucial role in growth development, signal transduction, various stress responses. To understand the biological functions banana GRAS gene family, genome-wide identification bioinformatics analysis was performed based on information from M. acuminata, balbisiana, itinerans genomic databases. In present study, we identified 73 MaGRAS, 59 MbGRAS, 58 MiGRAS genes bananas at whole-genome scale, 56 homologous were three genomes. Banana GRASs can be classified into 10 subfamilies, their structures revealed most gDNAs lack introns. The promoter sequences had large number cis-acting elements related to plant phytohormone, adversity responsiveness. expression pattern seven key members MaGRAS response low-temperature different tissues also examined by quantitative reverse polymerase chain reaction (qRT-PCR). microRNAs-MaGRASs target prediction showed perfect complementarity with five mac-miRNAs. all lowest roots, highest leaves during stress. MaSCL27-2, MaSCL27-3, MaSCL6-1 significantly lower under compared control, except for which slightly higher than 28°C control 4 h. dropped levels 24 h, 12 respectively. MaSCL27-4 MaSCL6-2 intermittently upregulated, rising 24h, while MaSCL22 less variable, remaining level small changes. summary, it tentatively hypothesized has an important function bananas. This study provides theoretical basis further analyzing resistance cold temperatures.