Characterization key genes of Arabidopsis seedlings in response to β-caryophyllene, eugenol using combined transcriptome and WGCN analysis

• DOI: 10.3389/fpls.2023.1295779
• Publication Date: 2024-01-04T04:48:23Z
• Authors: Yuqi Guo; Chang Liu; Yaran Zhang; Shuting Zheng; Ping Cao; Xiaomin Wang; Zengyuan Tian
• Keywords: Hemicellulose; Methyl jasmonate; Expansin

Weeds present a significant challenge to high crop yield and quality. In our study, we investigated the phytotoxic activity of β-caryophyllene (BCP) eugenol, which are natural allelopathic chemical compounds, on Arabidopsis seedlings. We found that these compounds inhibited growth thaliana plants. When either BCP or eugenol was applied, it led decrease in content cell wall components such as lignin, cellulose, hemicellulose, pectin; increase levels endogenous hormones like ETH, ABA, SA, JA Through transcriptome profiling, identified 7181 differentially expressed genes (DEGs) roots shoots were induced by eugenol. The involved synthesis pectin down-regulated, whereas related signal transduction up-regulated. However, IAA be down-regulated. Furthermore, characterized 24 hub using Weighted Correlation Network Analysis (WGCNA). Among them, 16 response primarily associated with hypoxia stress, while 8 linked inhibition division. Our results suggested had ability target multiple inhibit development Therefore, they can serve excellent candidates for biological herbicides.