Precocious cell differentiation occurs in proliferating cells in leaf primordia in Arabidopsis angustifolia3 mutant

• DOI: 10.3389/fpls.2024.1322223
• Publication Date: 2024-04-16T04:36:32Z
• Authors: Kazune Ezaki; Hiroyuki Koga; Noriko Takeda-Kamiya; Kiminori Toyooka; Takumi Higaki; Shingo Sakamoto; Hirokazu Tsukaya
• Keywords: Primordium

During leaf development, the timing of transition from cell proliferation to expansion is an important factor in determining final organ size. However, regulatory system involved this remains less understood. To get insight into system, we investigated compensation phenomenon, which number decreases while size increases organs with determinate growth. Compensation observed several plant species suggesting coordination between and expansion. In study, examined Arabidopsis mutant ANGUSTIFOLIA 3 ( AN3 )/ GRF-INTERACTING FACTOR 1 , a positive regulator proliferation, exhibits compensation. Though role has been extensively investigated, mechanism underlying excess an3 unknown. Focusing on early stage performed kinematic, cytological, biochemical, transcriptome analyses, found that had already increased during phase, active mutant. Moreover, at stage, chloroplasts, vacuoles, xylem cells developed earlier than wild-type cells. Transcriptome data showed photosynthetic activity secondary wall biosynthesis were activated proliferating These results indicated precocious differentiation occurs Therefore, suggest novel suppression expansion/differentiation phase.