The MADS-box genes SOC1 and AGL24 antagonize XAL2 functions in Arabidopsis thaliana root development

• DOI: 10.3389/fpls.2024.1331269
• Publication Date: 2024-03-21T15:28:45Z
• Authors: Claudio A. Castañ...; Maite Arrizubieta; Natalia Castelán-...; Diana Belén Sánch...; Carolina Caballer...; Estephania Zluhan...; Sandra C. Patiño-...; J.Arturo Arcinieg...; Berenice García-P...; María de la Paz S...; Elena R. Álvarez-...; Adriana Garay-Arroyo
• Keywords: MADS-box; Lateral root

MADS-domain transcription factors play pivotal roles in numerous developmental processes Arabidopsis thaliana. While their involvement flowering transition and floral development has been extensively examined, functions root remain relatively unexplored. Here, we explored the function genetic interaction of three MADS-box genes ( XAL2 , SOC1 AGL24 ) primary development. By analyzing loss-of-function overexpression lines, found that both critical components transition, redundantly act as repressors growth loss either or partially recovers growth, meristem cell number, production rate, length fully elongated cells short-root mutant xal2-2 . Furthermore, observed simultaneous leads to phenotypes, affecting number size, whereas is sufficient affect columella stem differentiation. Additionally, qPCR analyses revealed these exhibit distinct modes transcriptional regulation roots compared what previously reported for aerial tissues. We identified 100 differentially expressed by RNA-seq. Moreover, our findings expression certain involved differentiation, well stress responses, which are upregulated downregulated mutant, reverted WT levels absence