Solanum pimpinellifolium exhibits complex genetic resistance to Pseudomonas syringae pv. tomato

• DOI: 10.3389/fpls.2024.1416078
• Publication Date: 2024-10-23T05:21:02Z
• Authors: Jana A. Hassan; Nathan Diplock; Ilea J. Chau-Ly; Jamie Calma; Elizabeth Boville; Steven Yee; Taylor M. Harris; Jennifer D. Lewis
• Keywords: Pseudomonas syringae; Bacterial disease; Hypersensitive response

Pseudomonas syringae pv. tomato ( Pst ) is the causal agent of bacterial speck disease in tomatoes. The Pto/Prf gene cluster from Solanum pimpinellifolium was introgressed into several modern cultivars and provided protection against race 0 strains for many decades. However, virulent 1 that evade Pto -mediated immunity now predominate tomato-growing regions worldwide. Here we report identification resistance to a strain 19) wild accession S. LA1589 (hereafter LA1589), using our rapid high-throughput seedling screen. supports less growth than cultivars, does not exhibit hypersensitive response 19. We tested an existing set 87 Inbred Backcross Lines (IBLs) derived cross between susceptible lycopersicum E-6203 Using single-marker analysis, identified three genomic associated with resistance. Bacterial assays on IBLs confirmed these contribute planta . also mapped candidate genes var. cultivar Heinz BG-1706 LA1589. By comparing candidates two mapping approaches, were able identify 3 QTL 5 role This work will assist molecular marker-assisted breeding protect disease.