High-throughput phenotyping using hyperspectral indicators supports the genetic dissection of yield in durum wheat grown under heat and drought stress

• DOI: 10.3389/fpls.2024.1470520
• Publication Date: 2024-11-22T04:52:14Z
• Authors: Rosa Mérida-García; Sergio Gálvez; Ignacio Solís; Fernando Martínez...; Carlos Camino; Jose Miguel Soriano; Carolina Sansaloni; Karim Ammar; Alison R. Bentley; Victoria Gonzalez...; Pablo J. Zarco-Te...; Pilar Hernandez
• Keywords: Germ plasm; Anthesis; Plant Breeding; Candidate gene; Genome-wide Association Study

High-throughput phenotyping (HTP) provides new opportunities for efficiently dissecting the genetic basis of drought-adaptive traits, which is essential in current wheat breeding programs. The combined use HTP and genome-wide association (GWAS) approaches has been useful assessment complex traits such as yield, under field stress conditions including heat drought. aim this study was to identify molecular markers associated with yield (YLD) elite durum that could be explained using hyperspectral indices (HSIs) drought Mediterranean environments Southern Spain. HSIs were obtained from imagery collected during pre-anthesis anthesis crop stages an airborne platform. A panel 536 lines genotyped by sequencing (GBS, DArTseq) determine population structure, revealing a lack structure germplasm. material phenotyped YLD 19 six growing seasons at two locations Andalusia, southern GWAS analysis identified 740 significant marker-trait associations (MTAs) across all chromosomes, several common HSIs, can potentially integrated into Candidate gene (CG) uncovered genes related important plant processes photosynthesis, regulatory biological processes, abiotic tolerance. These results are novel they combine high-resolution imaging scale wheat. They also support tools identifying chromosomal regions response wheat, pave way integration