A real-world, cross-sectional, and longitudinal study on high-risk human papillomavirus genotype distribution in 31,942 women in Dongguan, China

• DOI: 10.3389/fpubh.2024.1409030
• Publication Date: 2024-08-01T13:14:50Z
• Authors: Huanxia Zhong; Wenwei Pan; Binbin Chen; Jiamin Gu; Yu Liang; Guoli Sun; Xinghua Huang; Huitao Yuan; Haina Guo; Ling Zhong; Zhuanfen Li; Ping Zhou; Siliang Zeng; Li Tang
• Keywords: Adult; China; Genotype; infection rate; Uterine Cervical Neoplasms; genotype distribution; Young Adult; 03 medical and health sciences; 0302 clinical medicine; Risk Factors; Prevalence; Humans; Longitudinal Studies; Early Detection of Cancer; Aged; screening; Papillomavirus Infections; Human Papillomavirus Viruses; Middle Aged; Cross-Sectional Studies; cervical cytology; Female; Public Health; Public aspects of medicine; RA1-1270; high-risk human papillomavirus

BackgroundPersistent human papillomavirus (HPV) infection remains a key risk factor for cervical cancer. HPV-based primary screening is widely recommended in clinical guidelines, and further longitudinal studies are needed to optimize strategies for detecting high-grade cervical lesions compared to cytology.MethodsFrom November 2015 to December 2023, 31,942 participants were included in the real-world observational study. Among those, 4,219 participants underwent at least two rounds of HPV tests, and 397 completed three rounds of HPV tests. All participants were tested for high-risk types of HPV 16/18/31/33/35/39/45/51/52/56/58/59/66/68 (hrHPV) and low-risk types of HPV6/11 genotyping. Some participants also received cytology or colposcopy with pathology.ResultsIn the cross-sectional cohort, the prevalence of hrHPV and all HPV subtypes was 6.6% (2,108/31,942) and 6.8% (2,177/31,942), respectively. The three top hrHPV genotypes were HPV52 (1.9%), HPV58 (0.9%), and HPV16 (0.9%). Age distributions showed two peaks at 45–49 and 60–65 years. For the primary screening cohort, the hrHPV prevalence rate increased from 4.8% in 2015–2017 to 7.0% in 2020–2020 and finally reached 7.2% in 2023. For the longitudinal cohort study, the hrHPV prevalence rates in the repeated population (3.9, 5.3, and 6.0%) were lower than the primary hrHPV screening rates (6.6%), which indicated that repeated screening might decrease the prevalence rate. Methodologically, the hrHPV (89.5%) and the screening group of 16 subtypes (92.3%) demonstrated superior sensitivity than the cytology group (54.4%). Moreover, the longitudinal study indicated that the persistent hrHPV subgroup had a significantly higher (p = 0.04) incidence of high-grade squamous intraepithelial lesions and more histology progression events (7/17 vs. 0/5) than the reinfection group.ConclusionThe study indicates a rising high-risk HPV prevalence in Dongguan, with repeated screening reducing this trend. The findings support HPV-based primary screening and might guide HPV vaccination and cervical cancer prevention in South China.